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Free eBook Clinical Applications of PCR (Methods in Molecular Biology) download

by Y. M. Dennis Lo

Free eBook Clinical Applications of PCR (Methods in Molecular Biology) download ISBN: 1588293483
Author: Y. M. Dennis Lo
Publisher: Humana Press; 2nd edition (March 1, 2006)
Language: English
Pages: 200
Category: Medicine
Subcategory: Medicine
Size MP3: 1418 mb
Size FLAC: 1697 mb
Rating: 4.5
Format: azw mobi txt mbr


This book is pitched at both pathologists and microbiologists who use PCR in a diagnostic setting.

The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. This book is pitched at both pathologists and microbiologists who use PCR in a diagnostic setting. It provides a user-friendly and relatively brief theoretical background to conventional and real-time PC. .

Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised. Clinical Applications of PCR offers an unprecedented collection of core PCR techniques for the study and diagnosis of human diseases

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised. Clinical Applications of PCR offers an unprecedented collection of core PCR techniques for the study and diagnosis of human diseases. Cutting-edge and essential for today's diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications exploiting the exquisite sensitivity of PCR in the detection of rare or single cells, as in identifying fetal cells circulating in maternal blood, preimplantation embryo diagnosis, or detecting circulating cancer cells.

Clinical Applications of PCR, Lo Y. M. Dennis. Варианты приобретения.

Goodreads helps you keep track of books you want to read. Start by marking Methods in Molecular Biology, Volume 336: Clinical Applications of PCR as Want to Read: Want to Read savin. ant to Read. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.

Reverse rase Chain Reaction (RT-PCR), R Kusec. Section 3: Clinical Applications. Detection of the Hepatitis C Virus by RT-PCR, CJ Healy and S Read

Reverse rase Chain Reaction (RT-PCR), R Kusec. PCR Amplification of Minisatellite DNA for the Detection of Mixed Chimerism After Bone Marrow Transplantation, E Roux. PCR Analysis of CD-44 Variants in Tumors, J Bolodeoku. Detection of the Hepatitis C Virus by RT-PCR, CJ Healy and S Read. The Application of PCR to the Detection of M. tuberculosis in Sputum Samples, M Maher, M Glennon, M Cormican, and T Smith. Microsatellite Analysis in Human Disease, J Koreth, CJ Bakkenist, and J O' D. McGee.

Methods in Molecular Biology™ is a trademark of The Humana Press In. One important question to resolve at the start of such an exploration concerns the name of the field at the intersection of statistics and molecular biology.

One important question to resolve at the start of such an exploration concerns the name of the field at the intersection of statistics and molecular biology. The term most widely employed by biologists, bioinformatics, seems quite inap-propriate.

Polymerase Chain Reaction (PCR). Speers DJ (2006) Clinical applications of molecular biology for infectious diseases. ClinBiochem Rev 27: 39-51. PCR was invented by Kary Mullis in 1983 and received Nobel Prize in Chemistry for the same. PCR can enzymatically amplify minute quantities of DNA or RNA to large number of copies (≥107 copies) in a short period hence provides ample target that can be readily detected. Clinical application: Allow subtype differentiation of bacterial isolates beyond the species and subspecies levels. Spoligotyping (spacer oligonucleotide typing). This technique is used as typing method for M. tuberculosis.

Methods in Molecular Biology. biology methods-just different DNA targets. like the polymerase chain reaction (PCR), has had a significant. How we measure 'reads'. The book covers nuclear and mitochondrial DNA typing. The authors describe fragment length analysis, both Southern. Blotting (VNTR analysis). and. PCR-based AmpFLPs.

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In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.